Applications
EtG / EtS from Urine on Luna Omega 5u Polar C18, 50x4.6mm
23842
Separation Mode: Reversed Phase
Reversed Phase
Polar C18
HPLC
Forensics/Toxicology
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

EtG / EtS from Urine on Luna Omega 5u Polar C18, 50x4.6mm
Analytes
1
2
3

Analyte not found

Details
LC Conditions (App ID: 23842)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic Acid in H2O
B: 0.1% Formic Acid in MeOH
Gradient Profile:
Step No.Time(min)%A%B
10982
224060
32.011090
431090
53.01982
64982
Flow Rate:
1 mL/min
Temperature:
25°C
Detection:
Tandem Mass Spec (MS-MS) @ 550°C
Sample Preparation Details
Description:
Strata-X-AW 33 µm Polymeric Weak Anion, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:
SPE Condition 1:
1 mL Methanol
SPE Condition 2:
1 mL 0.1% Acetic Acid in Water
SPE Load:
Load 100.00 µL Sample diluted in 0.1% Acetic Acid in Water
SPE Wash 1:
0.5 mL 50:50 Methanol:Water
SPE Dry:
5-10 min under high vacuum
SPE Elution 1:
0.6 mL 2% Ammonium Hydroxide in Methanol
SPE Elution 2:
0.6 mL 2% Ammonium Hydroxide in Methanol
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL Mobile Phase A

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