Product Information
Phase Detail
bioZen N-Glycan Clean-Up
bioZen Glycan sample preparation products offer targeted selectivity for N-glycan
bioZen N-Glycan Clean-Up
Easy Clean-up of Labeled, Released N-Glycans Using Solid Phase Extraction (SPE)

SPE HILIC stationary phase that excels at retention and recovery labeled released N-glycans. Microelution format allows for streamlined processing and clean-up of small sample volumes.

  • Small volume format allows for more concentrated samples
  • Minimal elution solvent
  • Excellent retention and recovery of labeled, released N-glycans
  • Faster clean-up methods to save on sample prep time

Brand
bioZen
Recommended Use
Extraction and clean-up of N-glycans
Protocol

Streptavidin Coated

Uniform magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules.

STEP 1

STEP 2

STEP 3

STEP 4

STEP 5
Start with Intact Glycoprotein Deglycosylation
Released N-Glycans by PNGase F in buffer solution
Labeling Glycans
2-AA or 2-AB for accurate and sensitive glycan detection 
Clean-Up
Removal of labeling reagent, salts, or surfactants
Analysis

96-Well Plate:
bioZen N-Glycan Clean-up
Part No.:
8M-S009-NGA
Condition:
200 µL Water
Equilibrate:
200 µL Water/Acetonitrile (15:85)
Load:
Labeled Sample (up to 400 µL of labeled sample)
Wash:
2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)
Elute:
3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)
Dilute:
100 µL Dimethylformamide and then 210 µL of Acetonitrile

bioZen LC Columns

bioZen Columns (mm) Biocompatible Guard Cartridges
  100 x 2.1 150 x 2.1 for 2.1 mm holder
bioZen 2.6 μm
Glycan
00D-4773-AN 00F-4773-AN AJ0-9800 AJ0-9000

Labeled Glycans from AGP


Sum of Peak Areas for Z-value Calculation

Glycan Sum of Peak Area (%) Multiplication Factor Charge Number Share
Asialo 0.28 0 0
Monosialo- 13.41 1 13.41
Disialo- 4.44 2 8.88
Trisialo- 52.49 3 157.47
Tetrasialo- 29.4 4 117.6
Total peak area 100% Z-value= 297.36